Substrate specificity of phosphoprotein phosphatase from spleen

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منابع مشابه

Purification and properties of phosphoprotein phosphatase from ox spleen.

Mangold, E. & Schmitt-Krahmer, C. (1927). Biochem. Z. 191, 411. McDonald, I. W. (1948a). Ph.D. Thesis, University of Cambridge. McDonald, I. W. (1948 b). J. Physiol. 107, 21P. McDonald, I. W. (1952). Biochem. J. 51, 86. McNaught, M. L. & Smith, J. A. B. (1947). Nutr. Abetr. Rev. 17, 18. Miller, J. I. & Morrison, F. B. (1942). J. Anim. Sci. 1, 353. Mitchell, H. H. & Hamilton, T. S. (1929). The B...

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Phosphoprotein Phosphatase of Soybean Hypocotyls: PURIFICATION, PROPERTIES, AND SUBSTRATE SPECIFICITIES .

A soybean histone-type protein kinase was used to prepare (32)P-labeled histone H1 as substrate for purification and characterization of a phosphoprotein phosphatase (EC 3.1.3.16) from soybean hypocotyls. The phosphatase has been purified 169-fold by ammonium sulfate fractionation, ethanol precipitation, and chromatography on Sephadex G-150, DEAE-Sephadex A-25 and Sephadex G-100. The activity o...

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Purification, properties, and substrate specificities of phosphoprotein phosphatase(s) from rabbit liver.

The phosphoprotein phosphatase(s) acting on muscle phosphorylase a was purified from rabbit liver by acid precipitation, high speed centrifugation, chromatography on DEAE-Sephadex A-50, Sephadex G-75, and Sepharose-histone. Enzyme activity was recovered in the final step as two distinct peaks tentatively referred to as phosphoprotein phosphatases I and II. Each phosphatase showed a single broad...

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A phosphoprotein phosphatase from ox brain.

1. The pyrophosphate-exchange reactions which are catalysed by rat-liver preparations and depend upon leucine or isoleucine are profoundly modified by 'soluble' ribonucleic acid and by changes in magnesium concentration. The preponderant influence is exerted by the terminal nucleotide sequence of the 'soluble' ribonucleic acid. Lysinedependent pyrophosphate exchange occurs only at relatively hi...

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Structural and functional basis of protein phosphatase 5 substrate specificity.

The serine/threonine phosphatase protein phosphatase 5 (PP5) regulates hormone- and stress-induced cellular signaling by association with the molecular chaperone heat shock protein 90 (Hsp90). PP5-mediated dephosphorylation of the cochaperone Cdc37 is essential for activation of Hsp90-dependent kinases. However, the details of this mechanism remain unknown. We determined the crystal structure o...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1959

ISSN: 0306-3283

DOI: 10.1042/bj0710537